Lipid Nanoparticle (LNP) In-vitro Services

Lipid nanoparticle (LNP) platforms move fast. Our in‑vitro capabilities help you de‑risk formulation decisions early, quantify potency and mechanism of action, and check safety signals before committing to in‑vivo studies. We combine physicochemical QC with cell‑based assays so your biological readouts are directly tied to the material you made.

Contact us to discuss project details.

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Physicochemical Analytics

  • Size & polydispersity index (dynamic light scattering); particle concentration; zeta potential
  • Morphology (TEM), osmolarity, pH, viscosity
  • Encapsulation efficiency and leakage/release kinetics (serum/pH stress)
  • Lipid composition & payload quantification (HPLC/LC-MS/fluorimetry)
  • Endotoxin (LAL) and sterility
Potency & Mechanism of Action

  • Transfection efficiency (luciferase/GFP reporters)
  • mRNA levels by RT-qPCR; protein expression by ELISA/Western
  • Endosomal escape assays (pH-sensitive probes; gelactin-recruitment reporters)
  • Uptake pathway mapping (clathrin/caveolae/macropinocytosis inhibitors)
  • Intracellular trafficking co-localization
Stability & Protein Corona

  • Serum/RNase stability and stress testing (freeze-thaw, pH, agitation)
  • Protein corona characterization (profiling on request
Safety & Immunotoxicity
  • Viability/cytotoxicity panels (XTT/MTT/CellTiter-Glo); ROS
  • Apoptosis/necrosis (Annexin V/PI)
  • Innate immune activation (PBMC cytokines multiplex)
  • Blood compatibility: hemolysis; complement activation (C3a, C5a, sC5b-9); platelet activation markers
  • PEG/anti-PEG interaction testing
Cell and Organoid-Based Assays

    The following cell and organoid-based assays can be used to test uptake, penetration, stability, efficacy and toxicity of LNP formulations.
  • Primary cells (PBMCs; hepatocytes; kupffer/macrophages; dendritic)
  • Standard immortalized lines (e.g., HEK293, HeLa, A549)
  • Organoid (e.g. liver, heart, kidney)
Throughput & Study Design

  • 96-well screening and confirmatory runs
  • Design of Experiments (DoE) for lipid ratios/N:P optimization
Functional Payload-Specific Assays

  • mRNA LNPs: Protein expression assay (ELISA, western blot, flow cytometry for the expressed protein)
  • siRNA LNPs: gene knockdown (qPCR, western blot)
  • CRISPR LNPs: genome editing efficiency (ICE, sequencing)