Human IL-12B
Interleukin-12B (IL-12B), or IL-12p40, is a crucial cytokine subunit involved in immune regulation. It combines with IL-12A (IL-12p35) to form IL-12, a key driver of Th1 responses, and with IL-23A (IL-23p19) to form IL-23, which influences inflammation and autoimmunity. Produced by dendritic cells, macrophages, and monocytes in response to infections, IL-12B activates the JAK/STAT pathway, promoting IFN-γ production, Th1 differentiation, and NK cell activity, while its role in IL-23 supports Th17 expansion and chronic inflammation.
Recombinant human IL-12B is frequently utilized in vitro to study immune signaling, Th1 polarization, and cytokine interactions. It is a valuable tool in cancer and autoimmune research, aiding in the development of targeted immunotherapies.
| Cat. No. | RC0012B |
|---|---|
| Purity | >95% in SDS-PAGE followed by coomassie staining |
| Endotoxin Level | <0.01 EU/µg (LAL Assay) |
| Source | Purified from the culture supernatant of HEK 293 cells genetically engineered to express human IL-12B protein with a C-terminal His tag. |
| Accession # | NP_002178 |
| Applications/Intended Use | Research Use Only: This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. |
| Molecular Mass | Predicted mass (34 kDa), appears ~45 kDa due to glycosylation |
| Formulation | Lyophilized from a 0.1 μm filtered solution in PBS with Trehalose |
| Reconstitution | Dissolve in sterile PBS to 0.1-0.5 mg/mL or user preferred stock concentration. |
| Shipping or Handling Information | Shipped at ambient temperature |
| CoA | Batch-specific test results will be provided with the product |
| Stability | Lyophilized: Stable for 12 months at -80°C; Reconstituted: Stable for 2 weeks at 4°C, or 3-6 months at -20°C |
| Disclaimer | All of TME Scientific cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. TME Scientific is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic or any other non-RUO application(s). |
