CRISPR Stable Knockout and Knockin Cell Line Model Generation Service
The Clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9 (CRISPR-Cas9) system has emerged as a versatile tool for gene editing and genome engineering. To understand gene function and regulation, it has been used in a wide variety of experimental models, including cell lines, laboratory animals, plants, and even in human clinical trials.
We offer state-of-the-art services for generating custom knockout cell lines targeting whichever gene(s) of interest you’re interested in. Additionally, gene knockout and gene tagging services are also offered at competitive price. At TME, we have a streamlined workflow for generating knockout and knockin cell line models with a fast turnaround time.
Features of our service
1. Cost-effective
2. Efficient
3. Fast
Interested in using our custom knockout or knockin cell line model generation service?. Please send us the details of your project to inquiry at info@tmescientific.com or contact us using the link given below, we will contact you with the details within one day.
CRISPR Stable Knockout Cell Line Generation Workflow
Service Details:
| Basic Service | CAT. NO. | Lead Time |
|---|---|---|
|
NURS.03 | Inquire |
| Basic Service | CAT. NO. | Price |
|---|---|---|
| A. Additional vials of cell line models (other than the number of vials indicated at the deliverable box below) | NURS.03A | $340/vial |
| B. Additional clones | ||
| C. Western blot evaluation (up to 1- clones) x | NURS.03B | $380/vial |
| D. Cell line authentication service | NURS.03C | $800/vial |
Deliverables:
1. 1 clone or 1 polyclonal pool, 2 vials (>1 x 106 cells/vial) of clones or pooled cells
2. Additional vials if more than 2 vials are required
3. Additional clones if more than 1 clone is required
3. Service report
It includes the following:
(a). Transfection details
(b). Killing curve data and single cell clonability analysis
(c). Screening & validation of knockout by
genomic cleavage detection assay and Sanger sequencing
(d). Test results indicating the presence or absence of microbial contaminants
(e). Applicable accessory service reports
Required Materials
1. Cells
At least 2×10⁶ cells per cell line, with passage number. Can be sent as frozen vials or live cells.
2. Media
Provide all necessary components for complete medium separately, along with clear instructions for reconstitution.
3. Flasks
If cells require specific cultureware, please include ≥5 coated 6-well plates and 10 T25 flasks.
4. Cell Morphology
Submit a cell image showing morphology prior to shipment. ABS will verify morphology upon receipt.
5. Knockout Validation
Standard methods include genomic cleavage detection assay and Sanger sequencing. If alternatives are preferred, please specify before placing the order.
How to Place an Order
Get a Quote
Complete the CRISPR Knockout and Knockin service requisition form and email it to info@tmescientific.com to receive a quote.
Place Your Order
Send an email to info@tmescientific.com including your quote number.
Receive Confirmation
You will receive an official order number via email once the order is processed.
Send Your Materials
Ship the required materials and include your order number on the package. Ensure cells are mycoplasma-negativexx.
We'll Handle the Rest
We will confirm cell morphology and mycoplasma status, and handle the remainder of the service.
Notes:
- This service requires a signed Material Transfer Agreement (MTA). The MTA does not grant commercial rights or IP ownership.
- A non-refundable deposit is required to initiate the service.
- x Customer must provide the antibody.
- xx Ensure cells are mycoplasma-negative at submission.