Stable Cell Line Model Generation Service

Generating stable cell line models involve several challenges, such as low transfection efficiency, long development times, labor-intensive processes and genetic variability.  These issues can complicate the process and affect the consistency and reliability of the resulting cell lines.

We offer state-of-the-art services for establishing stable cell line models for inducible and tunable transgene expression, gene knockout, gene knockin, gene up or down regulation  or other genome editing that meet your specific research needs.  At TME, we have a streamlined workflow for generating a cell line expressing your gene of interest with a fast turnaround time.

Types of stable cell line services offered

1. Inducible stable cell line models

2. CRISPR stable Knockout and Knockin cell line  models

3. CRISPRa/i cell line model

Interested in using our stable cell line model generation service? Please send us the details of your project to info@tmescientific.com or contact us using the link given below, we will contact you with the details within 1 day.

Service Details:

Basic Service Cat. No. Lead Time
  1. Cell line evaluation
    • Sterility test
    • Killing curve evaluation
    • Transfection test
    • Single cell clonability evaluation
  2. Vector construction
  3. Transfection
  4. Generation of polyclonal pools or clonal selection
  5. Expansion
  6. Cryopreservation and delivery.
 INQUIRE
Accessory Service Cat. No. Price
  1. Additional vials of stable cell line (other than the number of vials indicated at the deliverable box below)
  2. Additional clones
  3. Western blot evaluation (up to 10 clones) x
  4. Cell line authentication service

Deliverables:

1. 1 clone or 1 polyclonal pool, 2 vials (>1 x 106 cells/vial) of clones or pooled cells

2. Additional vials if more than 2 vials are required

3. Additional clones if more than 1 clone is required

3. Service report

     It includes the following:

     (a). Transfection details

     (b). Real-time PCR analysis of the transgene expression

     (c). Killing curve data and single cell clonability analysis

     (d). Test results indicating the presence or absence of microbial contaminants

     (e). Applicable accessory service reports

Required Materials

1. Cells

At least 2×10⁶ cells per cell line along with the passage number. Cells can be sent as either frozen vials or live cells.

2. Media

Submit all necessary components for making complete medium separately, along with clear instructions for preparation.

3. Flasks

If cells require specially treated dishes, please provide ≥5x coated 6-well plates and 10x T25 flasks.

4. Cell Morphology

Please include a picture of the cells showing morphology prior to shipment. TME will verify morphology upon receipt.

5. Transgene Expression Analysis

RT-PCR will be used to analyze transgene expression. Specify in advance if a different method is preferred.

How to Place an Order

1

Request a Quote

Complete the stable cell line model generation requisition form and email it to info@tmescientific.com.

2

Place Your Order

Email info@tmescientific.com with the quote number to initiate your order.

3

Receive Order Number

Once your order is placed successfully, you will receive an official order number via email.

4

Send Materials

Ship the required materials as outlined. Include the order number on the package.

5

We Take Care of the Rest

We will confirm morphology and mycoplasma status and complete the service process.

Notes:

  • This service requires a signed Material Transfer Agreement (MTA). The MTA confirms that materials are for research only and do not confer ownership or IP rights.
  • A non-refundable deposit is required to initiate the service.
  • x Customer must provide the antibody.
  • xx Please ensure cells are mycoplasma-negative at the time of submission.