Mycoplasma Detection and Decontamination Service
Mycoplasma is a widespread contaminant in cell culture with profound effects on cell growth and cell behavior. Mycoplasma is small in size, difficult to detect and hard to get rid of.
The conventional methods are effective on mycoplasma detection but they are time-consuming and usually take minimum of 28 days to obtain result. TME developed Nucleic acid Amplification Technique (NAT) is a PCR-based assay that is fast and reliable.
TME provides highly sensitive mycoplasma detection service (2-3 days) followed by decontamination with your request.
When you should perform mycoplasma detection:
You receive a new cell line from other laboratories.
Your cell has been passaged more than 10 generations.
Your cell is not growing actively as supposed to be.
Your cell is continuously growing for more than two months.
You are preparing a cell bank.
Cell growth condition is in doubt.
Please forward your request to info@tmescientific.com for questions or services.
Service Details:
| Basic Service | Unit | Cat. No. | Lead Time |
|---|---|---|---|
| 1. Mycoplasma detection | 1 Sample | ||
| 2. Mycoplasma decontamination | 1 Sample |
Deliverables:
Mycoplasma detection
1. Service Report
It includes the following:
(a). Service summary
(b). Test results indicating the presence or absence of mycoplasma
Mycoplasma decontamination
1. Service report
It includes the following:
(a). Detailed protocol
(b). Test results indicating the presence or absence of mycoplasma
(c). Image of the treated cell line before cryopreservation
2. 2 vials (>1 x 106 cells/vial) of mycoplasma free cells (cells tested negative for mycoplasma for 2 weeks)x
3. Additional vials if more than 2 vials are required
Required Materials
1. Cell Culture Supernatant
At least 0.5 ml of cell culture supernatant shipped with ice packs. Samples must have been cultured with cells for 48–72 hours. (Applicable for mycoplasma detection only.)
2. Cryopreserved Cells
Send at least 1×10⁶ cryopreserved cells on dry ice. Include all components needed to prepare 1 L of complete medium (base medium, serum, supplements, growth factors, etc.), any coating reagents required, and a full cell culture protocol.
3. Live Cells
We also accept live cells—please contact us directly for handling and shipping instructions.
How to Place an Order
Request a Quote
Complete the mycoplasma detection and/or decontamination service form at www.tmescientific.com or email your request to enquiry@tmescientific.com.
Confirm and Place Order
Once details are confirmed, send your invoice confirmation and quote number to enquiry@tme-scientific.com.
Receive Order Number
Once your down payment is received, an official order number will be emailed to you. Please ship your samples and related materials labeled with this order number.
Testing and Delivery
We’ll begin the test after confirming sample receipt. Initial results will be issued for your pending balance. Full service report will be sent upon final payment.